Purification and characterization of three elongation factors, EF-1 alpha, EF-1 beta gamma, and EF-2, from wheat germ.
نویسندگان
چکیده
منابع مشابه
Heat tolerance and expression of protein synthesis elongation factors, EF-Tu and EF-1a, in spring wheat
Protein elongation factors, EF-Tu and EF-1a, have been implicated in cell response to heat stress. We investigated the expression (accumulation) of EF-Tu and EF-1a in mature plants of spring wheat cultivars Kukri and Excalibur, and tested the hypothesis that cultivars with contrasting tolerance to heat stress differ in the accumulation of these elongation factors under prolonged exposure to hig...
متن کاملCloning and Characterization of EF-Tu and EF-Ts from Pseudomonas aeruginosa
We have cloned genes encoding elongation factors EF-Tu and EF-Ts from Pseudomonas aeruginosa and expressed and purified the proteins to greater than 95% homogeneity. Sequence analysis indicated that P. aeruginosa EF-Tu and EF-Ts are 84% and 55% identical to E. coli counterparts, respectively. P. aeruginosa EF-Tu was active when assayed in GDP exchange assays. Kinetic parameters for the interact...
متن کاملFrequency metrology on the EF 1
←X 1 g + 0,0 two-photon transition near 202 nm. For this purpose, the fourth harmonic of an injection-seeded titanium:sapphire pulsed oscillator is employed in a Doppler-free REMPI-detection scheme on a molecular beam of hydrogen. A frequency comb laser is used to perform the absolute frequency calibration on the continuous-wave CW laser that injection-seeds the oscillator. Chirp-induced freque...
متن کاملEf − Fe =
Let Uq(sl2) be the quantized enveloping algebra associated to the simple Lie algebra sl2. In this paper, we study the quantum double Dq of the Borel subalgebra Uq((sl2) ) of Uq(sl2). We construct an analogue of Kostant–Lusztig Z[v, v]-form for Dq and show that it is a Hopf subalgebra. We prove that, over an algebraically closed field, every simple Dq-module is the pullback of a simple Uq(sl2)-m...
متن کاملInteraction of eukaryote elongation factor EF 1 with guanosine nucleotides and aminoacyl-tRNA.
Evidence for two species of elongation factor 1 (EF 1(A) and EF 1(B)) from calf brain has been obtained by molecular sieve chromatography on Sephadex G-150. A high molecular weight form, EF 1(A), interacts with GTP to form an EF 1(A)-GTP complex. GDP also reacts with EF 1, but unlike the reaction with GTP, an EF 1(B)-GDP complex is formed that contains a lower molecular weight and labile specie...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1984
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)43456-9